|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
spty2d1
|
|
laevis
|
NF stage 13
|
prechordal plate
,
axial mesoderm
,
dorsal
|
btbd6
|
|
laevis
|
NF stage 13
|
axial mesoderm
,
dorsal
,
anterior
,
anterior neural ridge
|
gpr107
|
|
laevis
|
NF stage 13
|
prechordal plate
,
involuted dorsal mesoderm
,
axial mesoderm
,
dorsal
|
mob2
|
|
laevis
|
NF stage 13
|
involuted dorsal mesoderm
,
axial mesoderm
,
dorsal
|
ostc
|
|
laevis
|
NF stage 13
|
prechordal plate
,
axial mesoderm
,
dorsal
|
tmem52
|
|
laevis
|
NF stage 13
|
prechordal plate
,
axial mesoderm
,
dorsal
,
anterior
,
neural plate border
|
nudt22
|
|
laevis
|
NF stage 13
|
mesoderm
,
prechordal plate
,
involuted dorsal mesoderm
,
endomesoderm
,
anterior
|
|
|
FIG. 3. Isolated cDNA clones expressed in the AEM region and notochord. (A) Clone names and similar genes or proteins in the public databases are indicated on the left side of each panel. Results of whole-mount in situ hybridization for early neurula (stage 13) are shown in dorsal (left) and lateral (right) view. (B) Northern blot analysis. Clone names and their closest genes or proteins in the public databases are indicated on the left side of each panel and the deduced mRNA size (kb) of each clone on the right (see Table 2 for P11F3). The developmental stages are indicated on the top of each column by numerical figures according to Nieuwkoop and Faber (1967). 18S rRNA stained with ethidium bromide is for loading control. Ten micrograms of total RNA from stage-9, -10, -12.5, and -15 embryos were electrophoresed in each lane, blotted, and hybridized with probes derived from each clone except for crescent. Crescent probe was hybridized to the same blot as previously used (Hikasa and Taira, 2001) with a wider range of stages as indicated. |