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Fig 1. Expression analysis of sizzled.
In situ hybridization of stage 12 (A,B) and stage 18 (C,D) embryos revealed high levels of expression in both rostral and caudal ventral regions, as well as lower, but detectable expression within the neural ectoderm and neural crest. In later stages, sizzled in situ hybridization staining (E) was readily detected in the ventral and cranial regions. Standard RT-PCR (F) (stage 11) revealed readily detectable levels of sizzled RNA in whole embryos (WE), the ventral axial marginal zone (VAMZ) and dorsal axial marginal zone (DAMZ). Quantitative RT-PCR of the dorsal and ventral regions of stage 25 embryos (G) revealed readily detectable levels of sizzled RNA in both regions. qPCR analysis (H) of control and morpholino-injected embryos (both blastomeres of 2 cell embryos injected, embryos analyzed at stage 11) reveal an increase in sizzled RNA snail2/slug, snail1, and twist1 morphant embryos.
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