| |
Fig. 6. Identification of TAAR gene expression in the olfactory organ and
brain of larval and adult X. laevis. RT-PCR analysis of TAAR 1 and TAAR 2
(for detailed information see Methods) mRNA in the olfactory organ and brain
of larval (A) and adult (B) X. laevis. The 285-bp and 251-bp products represent
X. laevis TAAR 1 and TAAR 2, respectively. Control GAPDH (233 bp) and
beta-actin (191 bp) RT-PCR reactions are shown. (A) L, DNA-Ladder; 1,
GAPDH (olfactory organ); 2, GAPDH (brain); 3, TAAR 1 (genomic DNA); 4,
TAAR 1 (olfactory organ); 5, TAAR 1 (brain); 6, TAAR 2 (genomic DNA); 7,
TAAR 2 (olfactory organ); 8, TAAR 2 (brain). (B) L, DNA-Ladder; 1, betaactin
(middle cavity); 2, beta-actin (principal cavity); 3, beta-actin (brain); 4,
TAAR 1 (middle cavity); 5, TAAR 1 (principal cavity); 6, TAAR 1 (brain); 7,
TAAR 2 (middle cavity); 8, TAAR 2 (principal cavity); 9, TAAR 2 (brain). |
