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Fig. 5. Xdm mutants fail to express tbx5 in the forelimb-forming region. (A–C) WISH analysis of stage 48 wild type embryos detects tbx5 in the nascent forelimb bud and heart (white arrow). (D–F) Xdm embryos lack tbx5 expression in equivalent flank region. (B, E) Magnified view of boxed regions in (A) and (D) showing pronephros (black arrow). (C) 10× view of a transverse section through wild type flank showing tbx5 expression in the nascent forelimb (gray arrow) below the pronephros (black arrow). (F) 10× view of a transverse section of xdm at equivalent level of the forelimb region shows pronephros (black arrow) but no tbx5 expression. |
