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Figure 3. Whole mount in situ hybridization analysis of pfdn6a and tcp1α during
neurulation. (A-C, F-H) show frontal views (dorsal to the top) of hybridized embryos at the
indicated neurula stages; white arrowheads indicate neural crests. (D, I) Dorsal view of stage 18/19
embryos injected unilaterally with Rx1 RNA and nuclear LacZ RNA as a tracer (red staining).
Arrows point at regions displaying stronger repression. (E, J) Lateral views of hybridized stage 22-
23 embryos; anterior is to the left; nc: neural crests. Scale bars = 250μm. |