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FIG. 4. In situ hybridizations to
sections through fundic regions of
X. laevis stomach. A, 8, C, hybridi7.a·
tion with ' H-labeled single-stranded
cRNAs representing xP1 (from eDNA
clone pXSP-3' ·4.1}; exposure time, 14
days. D, E, F, hybridization with 32PIabeled
single-stranded cRNAs repre·
senting xP4 (from eDNA clone pXSP·
5' -1.26, similar pXSP-5' -1.27); exposure
time, 5 days. Positive signals (dark/ieki
illumination) were obtained with the an·
tisense probes (A, D), but not with the
sense probes (C, F). 8, E, corresponding
phase contrast pictures to A and D. Scale |