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FIG. 5. Ventral-to-dorsal progression through a stage 46 Xenopus heart. Images were digitally created from a series of 48 optical sections (taken 5um apart) through a double-labeled stage 46 Xenopus heart (red, CT3; green, JB3). Image A represents the most ventral 8 images of this series, digitally merged; image B, the next 8, etc. The relative positioning of heart structures to one another, as well as an individual structure order of emergence within the series, can be established: the truncus arteriosus and ventricle are always most ventral (A). Progressing dorsally, the spiral valve (B) appears within the truncus. Next, the single atrioventricular valve (C) is seen overlying the atrial septum (D and E). Midway through the series (C and D), the first views of the atria, as well as the aortic branches, are viewed. Both atria and the sinus venosus are seen more dorsally (D and E). Finally, the back wall of the atria is seen, positioned most dorsal (F). Scale bar, 100 um. |
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FIG. 6. Early vasculature in Xenopus. Ventral view of a double-labelled stage 46 Xenopus embryo (red, anti-cardiac tropon in T (CT3); green, anti-type VI collagen). Image represents 45 optical sections through the embryo, each 10 um thick, digitally merged and colored. The first three branches on the left side of the paired aortic arches are easily distinguished with the antibody to type VI collagen, as are all chambers and the truncus arteriosus with the antibody to cardiac troponin T. Scale bar, 100 um |
