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FIG. 1. Proapoptotic proteins
Bax and p53 are sequestered in
Xenopus oocyte nucleus and
excluded from the mitochondrial
cloud. (A, B, D, F, G) Whole-mount
and (C, E, H) section immunostaining
of Stage I–II (A, F) albino,
and (B, C and G, H) and Stage IV
(D, E) wild type oocytes with Bax
antibody. In (A–C) control and
(F–H) heat-shocked Stage I–II
oocytes, Bax protein is concentrated
in the nucleus and excluded
from the mitochondrial cloud
(arrows in B, C, H). Arrow in F
points to the heavily damaged
oocyte with cytoplasmic blebbing.
In Stage IV oocytes Bax is
exluded from the nucleus and
concentrated (arrow) in perinuclear
region (D, E). Whole-mount
(I) control and (J) heat-shocked
oocytes immunostained with p53
antibody showing the protein concentrated
in the nucleus and
excluded from the mitochondrial
cloud (arrows). The level of p53
expression seems to be similar
in control and heat-shocked oocytes,
the oocyte in panel J showing
lower than control expression
of p53 was chosen just to clearly
visualize the mitochondrial cloud.
N, nucleus. Scale bars represent
50 lm in A–C and F–J, 200 lm in
D and 75 lm in E. |
