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Fig. 2. ?Np63 loss and gain of function results in anterior patterning defects in Xenopus laevis. (A) Four-cell Xenopus embryos were injected dorsally with ?Np63alpha or ?Np63alpha-R304W mRNA (2 ng each) and scored at the tailbud stage. (B) Four-cell Xenopus embryos were injected dorsally with p63MO-A (200 ng) in the presence or absence of ?Np63alpha mRNA (2 ng), and scored at the tailbud stage. (C) Table summarizing results from embryo injections in (A) and (B). (D) Animal caps injected with control or p63MO, or ?Np63-encoding RNA were fixed, sectioned, and stained for p63 (4A4 antibody). (E) Whole embryo RT-PCR shows that the pan-mesodermal marker Xbra and the anterior mesodermal marker Gsc are down-regulated in ?Np63alpha-injected but not ?Np63alpha-R304W-injected embryos. (F) In situ hybridization analysis shows that ?Np63 is expressed in the animal pole of late blastula (stage 9) embryos and in most of the epidermis of mid-neurula (stage 18) embryos (anterior is on the left).
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