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Fig. 2. Expression profile of Xtrb2 transcripts during early development of Xenopus laevis. (A) Expression profile of Xtrb2-S, Xtrb2-L, and Xbra at stages from fertilization to stage 24. The numbers are normalized against plakoglobin expression. (B–E) Whole mount in situ hybridization of Xtrb2-S (B, D) and Xtrb2-L (C, E) at stage 9 (B, C) and stage 10.5 (D, E). (F) Relative expression levels of Xtrb2-L in the three germ layers at stage 9 assayed by quantitative RT–PCR. Xtrb2-L is highly expressed in ectoderm and mesoderm, while sox17 and Xbra were expressed predominantly in endoderm and mesoderm, respectively. Data are normalized against the amount of input RNA. |