| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| ttc25 | xenopus | gastrocoel roof plate |
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Experiment details for odad4
RFX2 is broadly required for ciliogenesis during vertebrate development.RFX2 is broadly required for ciliogenesis during vertebrate development.
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| Fig. 7. TTC25 is expressed in ciliated tissues. WMISH shows that TTC25 is expressed in the GRP (A, A′, B′), epidermis (B, C), neural tube (C, C′, D), otic vesicle (E, arrow), and kidney tubule nephrostomes (E, arrowhead). A and C are dorsal views, anterior up. A′ and B′ are sagittally-bisected embryos shown in A and B, respectively. B and E are lateral views, anterior to the left. C′ and D are transverse sections showing the dorsal midline. | ||||||||||||||||
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| Fig. 6. Rfx2 is required for the expression of a ciliary gene TTC25, but not α-tubulin. (A)–(D) Rfx2 morpholino is injected into one ventral blastomere at the 4 cell stage. (A) α-tubulin in situ hybridization on the control side of epidermis. (B) α-tubulin in situ hybridization on the Rfx2 morpholino-injected side. α-tubulin expression is not significantly changed (also see suppl. Fig. 4). (C) TTC25 expression patterns on control epidermis. (D) TTC25 in situ hybridization on Rfx2 morpholino-injected side of epidermis. TTC25 expression is reduced in epidermal multi-ciliated cells. (E)–(H) Rfx2 morpholino is injected into both dorsal blastomeres at the 4 cell stage. (E) Dorsal view of TTC25 expression on neural plate of a control embryo. (F) Dorsal view of TTC25 expression on neural plate of an Rfx2 morphant. TTC25 expression is reduced. (G) Sagittal section view of a control embryo. TTC25 is expressed in the gastrocoel roof plate (GRP). (H) Sagittal section view of a Rfx2 morphant. TTC25 expression is reduced in GRP. |