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Fig. 5. MTOC activity of centrioles. Whole mount of froglet ovary stained with anti-tubulin antibody (A and B). Microtubules (green, arrows) are visible in the
basal parts of cystocytes and traversing the bridges between cystocytes (arrowheads) (A). Asters and spindles (arrows) in mitotic cystocytes (B). Electron
microscopy image of microtubules in cystocytes (C– E). Microtubules (arrows) branching toward the mitochondria (m) in the PMC region of the cystocyte.
Fragment of the nucleus is visible in the upper left corner (C). Numerous microtubules (arrows) are visible in the PMC region. The centriole (thick arrow) is at
the center of the PMC between mitochondria (m) and mitochondrial cement (thick short arrows) (D). Fragment of PMC with the microtubules (arrows)
radiating from the centriole (short arrow) toward the mitochondria (m) (E). Scale bar is equal to 14 Am in A and B, 300 nm in C and D, and 200 nm in E. |