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Fig. 5.
Protein detection of prune and NM23 in Xenopus retina.
(A–B) Immunofluorescence for protein detection of prune using A59 antibody, NM23 using K73 antibody and tubulin (anti-tuj1 antibody) on retinal sections at stage 24 (panel A) and stage 32 (panel B). DAPI was used for counterstaining (blue) and, as control, staining with only secondary anti-rabbit antibody on retinal sections at stage 32 (Control). (C) Immunofluorescence of retinal sections at stage 41 of development with antibodies against prune (A59), NM23 (K73), tubulin (anti-tuj1 antibody), and GCL marker (anti-islet antibody). DAPI was used for counterstaining (blue) (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.). |
