| |
Fig. 8. Immunofiuorescence microscopy performed on cryostat sections of frozen ovarian tissue from Xenopus laevis using
monoclonal antibody VIM-3B4. (A,B) The same field is shown in epifluorescence (A) and phase-contrast (B) optics. Note
that only the interstitial cells show a bright fluorescence, whereas the oocytes are negative. (C,D) In a region with
previtellogenic oocytes (C, epifluorescence; D, phase contrast) the oocytes are negative whereas the interstitial cells, the
endothelial cells and the erythrocytes of the blood vessel show intense fluorescence, v, blood vessel. Bars. 50um. |
