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Fig. 9. Vimentin staining in the stage-35 embryo. In a lateral optic section of stage-35 embryo stained with RV202 (A), vimentin can be found in the neural tube, in the dorsal fin (df) and on the somites (the somitic mesenchyme is mostly out of focus in this picture). Vimentin is expressed in the pronephric mesenchyme (pm) below the first six somites with progressively less mesenchyme below the more posterior somites. The reticulated pattern of the vitelline veins (vv) can be seen throughout the belly around the yolky endoderm. Vimentin-expressing mesenchyme is present on the branchial arches (ba), around the otic vesicle (oi), covering the eye (e) and on the surface of the brain (b). A close-up optical section of the dorsal fin (B) reveals that the morphology of individual dorsal fin mesenchymal cells (dm) can be distinguished by the vimentin staining. In a lateral optical section midway through the embryo (C), vimentin-expressing mesenchyme surrounds the heart in the pericardial cavity (cm). A close lateral optical section of the anterior somites (D) reveals individual, vimentin-expressing mesenchymal (sm) cells. Individual pronephric mesenchymal cells (pin) are visible below the somites. A close lateral view of the region ventral to the anterior somites is shown in D. (E) reveals that the pronephric mesenchyme (pm) expresses vimentin, as do cells of the vitelline veins (vv). b, brain; ba, branchial arch; c, cement gland; df, dorsal fin; e, eye; nt, neural tube; ot, otic vesicle; s, somite; vv, vitelline veins. Bars, 250um in A, 50um in B, 20um in C, 100um in D and 20um in E. |
