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Fig. 4. DMg, vimentin, and glial fibrillary acidic protein (GFAP) localization on stage 45/46 hindbrain. A: Hoechst nuclear staining. Arrowheads indicate the rhombomere centers. B: DMg-positive radial glial processes are observed throughout the hindbrain. Segmental distribution of radial glia, if any, is not evident. C: Vimentin-positive cells start arranging in a segmental manner. D: GFAP-positive cells are extensively colocalized with vimentin, except the longitudinal fibers are strongly positive for GFAP (arrow). Scale bar 5 20 mm in A (applies to A–D). |
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Fig. 5. DMg mRNA, DMg, vimentin, and glial fibrillary acidic protein (GFAP) protein localization in stage 53/54 hindbrain longitudinal sections. A: DMg transcript is concentrated in the center of rhombomere (arrowheads). B: In contrast, DMg-positive radial glial processes distribute in the boundaries of the rhombomere and are occasionally seen in the center region. C: Vimentin-positive radial glial processes distribute in a similar manner as that of DMg. D: However, GFAP immunoreactivity is strongly concentrated in the center of each rhombomere and distribute throughout the white matter. Scale bars 5 20 mm in A–D. |
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Fig. 6. A: DiI is injected into the ventricle of the stage 53/54
hindbrain. DiI uptake is exclusively seen in the radial glia at the
centers (A). B: These processes are almost exclusively derived from
the region where DMg-positive processes are found. The processes of
the boundary radial glia extend upward in a fan-shaped manner (B).
C,D: Vimentin and glial fibrillary acidic protein (GFAP) localization
in stage 60 hindbrain. Vimentin is mainly detected at the borders (C),
whereas GFAP is localized in the center (D). Arrowheads indicate
center regions. Scale bars 5 20 mm in B (also applies to A) and D (also
applies to C). |
