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Figure 3. Expression of Xenopus laevis Coxsackievirus and Adenovirus Receptors (xCAR) mRNA in embryonic and tadpole stages. A: Reverse transcriptase-polymerase chain reaction (RT-PCR) of xCAR1 and xCAR2 in mRNA extracted from whole embryos at the developmental stages indicated. xCAR1 is expressed from gastrulation onward, while xCAR2 is expressed both maternally and zygotically. B: RT-PCR of xCAR1 and xCAR2 in mRNA extracted from stage 54-56 tadpole tissues. M, muscle; Li, Liver; H, head; S skin. C-H: Whole-mount in situ hybridizations. C,D show no probe controls at stage 25 and 31; E,F show xCAR1 at stage 25 and 31; G,H show xCAR2 at stage 25 and 31. White arrowheads in G and H indicate expression of xCAR2 in dorsal tail bud and in pronephros and nephric duct, which are greater than seen for xCAR1. |
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vsig8 (V-set and immunoglobulin domain containing 8) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 31, lateral view, anterior left, dorsal up. |
