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Fig. 3. In situ hybridization of xWt1 to Xenopus embryos. The embryos are not cleared. Except in A and C, which are dorsal views with anterior to the left, all panels are lateral views with anterior to the left. A: Stage 20. The white arrows indicate xWTf specific staining. 6: Stage 23-24 embryo. C: Stage 23-24 embryo dorsal view. D: Stage 27-28 embryo. The white arrow indicates xWt1 staining. E: Stage 30 embryo. F: Enlargement of the pronephric region of panel E (boxed). G: Stage 39
embryo, close-up of the head region. Note the small blue patch of stain behind, and much smaller than the pronephros itself. This corresponds to
the glomus (red arrow). The black arrow indicates heart staining. H: Pronephric tubules of a stage 36-37 tadpole visualized by staining with monoclonal antibody 3G8. Note the difference between the position and shape of the tubules (red arrow, H) and the glomus (red arrow, G). |
