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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
ywhaq
|
|
laevis
|
NF stage 25
|
cranial ganglion
|
ywhaq
|
|
laevis
|
NF stage 40
|
fin
,
brain
,
spinal cord
,
cranial ganglion
,
epidermis
,
[+]
|
ywhah
|
|
laevis
|
NF stage 25
|
|
ywhah
|
|
laevis
|
NF stage 40
|
head
|
ywhag
|
|
laevis
|
NF stage 25
|
|
ywhaz
|
|
laevis
|
NF stage 25
|
brain
|
ywhaz
|
|
laevis
|
NF stage 40
|
fin
,
brain
|
ywhae
|
|
laevis
|
NF stage 25
|
brain
,
paraxial mesoderm
,
head
|
ywhae
|
|
laevis
|
NF stage 40
|
fin
,
brain
,
spinal cord
,
cranial ganglion
,
epidermis
,
[+]
|
ywhab
|
|
laevis
|
NF stage 25
|
tail bud
,
paraxial mesoderm
,
proctodeum
,
head
,
pharyngeal region
|
ywhab
|
|
laevis
|
NF stage 40
|
spinal cord
,
proctodeum
,
pronephric kidney
,
head
,
pharyngeal region
|
|
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Figure 3. In situ hybridization staining reveals characteristic spatial expression patterns of six 14-3-3 family members in normal developing embryos. A,B: Normal stage 25 (A) and stage 40 (B) embryos were probed with digoxigenin-labeled 14-3-3 family member-specific RNA probes and were visualized by anti-diaminobenzidine alkaline phosphate staining to determine the spatial expression patterns of 14-3-3 gamma (a), epsi (b), tau (c), zeta (d), eta (e), and beta (f). A: The 14-3-3 gamma was highly abundant in the cranium and central nervous system. The 14-3-3 gamma (a), epsi (b), and tau (c) were all abundantly expressed on the body surface, but 14-3-3 zeta (d), eta (e), and beta (f) were not. B: At stage 40 (a), 14-3-3 gamma was highly abundant in the cranium and nervous tissues compared with other 14-3-3 family members; b: 14-3-3 epsi and tau were both abundantly expressed in the skeletal myotomes as well as the posterior trunk and tail fin regions; d: 14-3-3 zeta was expressed peripherally in the translucent tail fin but not in the trunk of the embryo; e,f: in contrast to zeta , 14-3-3 eta and beta were preferentially expressed in the trunk but not in the tail fins. C: Magnified views showing the expression of various 14-3-3s in the cranium at stage 40. |
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ywhag (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 40, lateral view, anterior left, dorsal up. |
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ywhag (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 40, lateral view, anterior left, dorsal up. |