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Figure 3. ALK4 distribution in gastrula stage embryos. A,C,E,G,J: Embryos were collected at stages 10 (A), 11 (C), 12 (E), and 13 (G) and whole-mount hybridized with either an ALK4 antisense probe (A,C,E,G) or ALK4 sense probe (J). B,D,F,H,I: Hybridized embryos were sectioned along the animal-vegetal axis (B,F), transversely (D,H), or sagittally (I,J). ALK4, activin-like kinase-4; ARC, archenteron; BC, blastocoele; BP, blastopore; DM, dorsal mesoderm; ECT, ectoderm; ENDO, endoderm; IDM, involuting dorsal marginal zone; IVM, involuting ventral marginal zone; NTC, notochord; PM, paraxial mesoderm; PCP, prechordal plate; VM, ventral mesoderm. |
