| |
Fig. 3. Representative sections showing PACAP mRNA distribution
in stage 26–28 embryos. Embryos were sectioned after wholemount
in situ hybridization. PACAP mRNA was not detected in the
forebrain or anterior rhombencephalon (open arrows in A–C) but was
found to be present from the middle portion of the rhombencephalon
(arrows in D) and throughout the spinal cord (arrows in E–H). Outside
the neural tube, PACAP gene expression was also noted in the
trigeminal ganglia (arrows in B,C). Dashed lines in C–H outline the
boundary of the neural tube. N, notochord; M, mesencephalon; P,
prosencephalon; R, rhombencephalon; T, tuberculum posterior; SC,
spinal cord. Scale bar 5 500 mm in A (applies to A,B), 100 mm in H
(applies to C–H). |
