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Fig. 3. Sections of the midneurula stage embryos stained for XAgr2 (A,C) and Xanf-1 (B,C) expression. (A) A sagittal section of XAgr2 expression at the midneurula stage. XAgr2 expression is located in the superficial layer of the anterior ectoderm. The arrow indicates the dorsal limit of expression. (B) Xanf-1 expression at the midneurula stage. The arrow indicates the limit of expression in superficial cells, while expression in the deep cells extends more anteriorly. (C) Double in situ hybridization for XAgr2 (shown in red) and Xanf-1 (shown in blue) at the midneurula stage. (D) Scheme of the XAgr2 expression region (shown in red) and the Xanf-1 expression region (shown in blue) in the superficial and deep layers of anterior ectoderm at the midneurula stage. |
