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FIGURE 3. Expression pattern of xAmer2 in Xenopus embryos by whole-mount in situ hybridization. A, xAmer2 expression in Xenopus embryos at NF stage 10.5 in the animal hemisphere (lateral view, animal pole to the top). B, xAmer2 expression in the anterior neural plate at NF stage 16 (dorsal view, anterior to the top). C, xAmer2 expression in the central nervous system, otic vesicle (o), eye (e), and cranial ganglia and nerves (arrowheads; V, trigeminal; VII, facial; IX, glossopharyngeal; X, vagal) at NF stage 35 (lateral view, anterior to the left). C′, cross-section through the head region of an NF stage 35 embryo (anterior to the left). xAmer2 expression was clearly seen in the forebrain (fb), midbrain (mb), and hindbrain (hb) but not the boundaries between these domains (arrowheads). |
