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Fig. 3. Comparison of anoctamin expression in X. laevis by reverse transcriptase (RT)-PCR and real-time PCR. Each expression level of anoctamins was normalized with β-actin used as internal control. (A) Bar graphs of RT-PCR. One round of PCR was carried out with 38 repeats to determine the specific products of xANO2, xTMEM16A, and β-actin gene. Each specific product of xANO2, xTMEM16A, and β-actin gene was amplified by RT-PCR. The β-actin gene was used as an internal control. Lanes 1–9 represent brain, heart, lung, liver, small intestine, colon, kidney, spleen and oocyte, respectively. (B) Quantitative histogram of real-time PCR. Real-time PCR was carried out with 50 repeats for quantitative analysis for anoctamin expression in X. laevis tissues. (C) Tissue distributions of anoctamin mRNA expression. Quantitation of anoctamin is shown as a bar graph compared to RT-PCR.
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