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FIG. 8. The expression of a1 subunit protein during neurulation. (A) Stage 13. The rostral notochord (no) begins to stain for a1 subunit
protein. The neural plate (NP) and somites (som) show little stain. (B) Stage 14, rostral. The notochord begins to show prominent staining
for a1 subunit protein. (C) Stage 14, caudal. Notochord staining (no) is absent. There is some patchy staining elsewhere. som, somites.
(D) Stage 17. The deep surface of neural plate cells (NP) stain for a1 subunit protein. The notochord stains intensely and the somites (som)
show only weak and patchy stain. (E) Stage 17. The boundary (arrow) of a1 subunit protein between deep neural plate (NP) and lateral
ectoderm (LE) cells. Part of the intensely stained notochord is visible and the somite (som) shows little stain. (F) Stage 17: an example of
a1 subunit protein expression in floor plate cells lying immediately above the intensely staining notochord. (G) Stage 17. Negative control.
NP, neural plate; no, notochord; som, somite. Bars, 25 mm for F, G; otherwise, 100 mm. |
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FIG. 9. The expression of a1 subunit protein after closure of the neural tube. (A) Stage 22/23. Note intense stain of notochord, floor plate
(FP), and apical surfaces of neural tube cells (NT). som, somites. (B) Enlargement of A to show the region just above the notochord to
further illustrate floor plate (FP) and neurocoel lining stain. NT, neural tube. (C) Ventral region of same section to show pattern of a1
subunit protein expression in the expanding archenteron (arch). The ventral side of the notochord is just visible. The somites (som)
continue to show little expression. (D) Stage 36. The dorsal notochord and ventral region of the neural tube (NT). Note marked expression
along the cell surfaces lining the neurocoel, particularly in the floor plate. (A, C) Bar, 100 mm; (B, D) Bar, 25 mm. |
