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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
slc4a4
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
olfactory placode
,
otic vesicle
,
forebrain
,
pronephric duct
,
pronephric kidney
,
[+]
|
slc12a1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
pronephric duct
,
pronephric kidney
,
pronephric tubule
,
proximal tubule
|
lhx1
|
|
laevis
|
NF stage 25
|
hindbrain
,
spinal cord
,
pronephric mesenchyme
|
rgn
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
glomus
,
pronephric kidney
|
wt1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
glomus
,
pronephric tubule
,
proximal tubule
|
wt1
|
|
laevis
|
NF stage 25
|
glomeral mesenchyme
,
pronephric mesenchyme
,
glomus
|
hnf1b
|
|
laevis
|
NF stage 25
|
posterior neural tube
,
pronephric mesenchyme
|
hey1
|
|
laevis
|
NF stage 25
|
brain
,
forebrain
,
midbrain
,
hindbrain
,
neural crest
,
[+]
|
pax2
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
otic vesicle
,
midbrain-hindbrain boundary
,
spinal cord
,
pronephric nephrostome
,
pronephric duct
,
[+]
|
pax2
|
|
laevis
|
NF stage 25
|
spinal cord
,
post-anal gut
,
optic vesicle
,
pronephric mesenchyme
,
lateral plate mesoderm
,
[+]
|
nphs1
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
glomus
|
cldn19
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
pronephric nephrostome
,
pronephric duct
|
mecom
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
forebrain
,
midbrain
,
hindbrain
,
pronephric duct
,
cranial neural crest
,
[+]
|
mecom
|
|
laevis
|
NF stage 25
|
forebrain
,
telencephalon
,
mandibular crest
,
neural crest
,
pronephric mesenchyme
,
[+]
|
cimap1a
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
epidermis
,
pronephric nephrostome
|
clcnkb
|
|
laevis
|
NF stage 33 and 34
to
NF stage 35 and 36
|
pronephric duct
,
pronephric tubule
,
proximal tubule
,
distal tubule
|
|
|
Supplementary Material. Fig. S2 Pronephric phenotype induced by injection of MoPax2.2. Whole mount in situ hybridization for tubule and glomus marker genes. Embryos were injected with MoPax2.2 at the 4-cell stage, in the equatorial region of both left blastomeres. They were cultured until the tailbud stages 3335 (A) or 25 (B) and analyzed by in situ hybridization with specific probes for the indicated genes. For each embryo, injected (aq) and uninjected (aq) sides are shown. Pax2 depletion does not lead to any change in the expression of pronephric marker genes at stage 25. At the late tailbud stage, gene expression in the proximal part of the tubule, including nephrostomes, is affected by MoPax2.2 while expression in the distal tubule is not modified except for the terminal differentiation marker clcnkb1 whose expression is completely inhibited. Expression of the glomus marker genes wt1 and nephrin is not significantly affected in MoPax2.2 injected embryos. Insets show higher magnification of the anterior pronephric territory. Numbers indicate how many embryos showed the phenotype among the total number of injected embryos. |