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Figure 3. xlbax mRNA spatiotemporal expression during natural metamorphosis. B,D: Endogenous bax mRNA expression was followed in tail muscle at stage (St) NF55 (B) and NF62 (D) by in situ hybridization, using an antisense digoxigenin-labeled probe on longitudinal Vibratome sections. A,C: Some sections were hybridized with a sense probe to test for specificity (A, NF55 and C, NF62). Black arrows indicate dead muscle fibers. The in situ hybridization was done twice on independent groups of tadpoles and gave identical results. |
