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Fig. 6. In situ hybridization analysis for putative Pitx3 target genes Obscnl and Baz2b. Visual comparisons of gene expression patterns between control-morpholino (Cmo) and Pitx3-morpho- lino (Pmo) right side-injected embryos. A: Obscnl shows a loss of expression in the branchial arches (black arrow), otic vesicle, and retina when treated at stage 27 with Pmo (A0) versus Cmo (A). B: Baz2b is substantially reduced in response to Pmo (B0) in the retinal layer of the optic protuberance (white arrow), as well as in the pronephros and in the anterior region of the dorsal axis, when compared with Cmo (B). |
