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Fig.2. Expression of Bmp7 mRNA in the Xenopus retina during development. (A,B) Xenopus embryos processed for wholemount in situ hybridization with Bmp7 antisense riboprobe at stages 26/28 (A) and 32 (B). (C) Transverse section through the central portion of the optic vesicle of a stage 26/28 embryo labeled for Bmp7 mRNA (see line 1 in A). (D) Transverse section through the peripheral retina of stage 26/28 embryo (line 2 in A) reveals expression of Bmp7 mRNA along the entire dorsoventral axis. (E) Transverse section through a stage 32 embryo shows Bmp7 expression in the dorsal and ventral CMZ (black arrowheads). (F) A patch of Bmp7 expression (arrow) appears in the mesenchyme ventral to the EF retina at stage 35/36. Anterior is to the left in panels (A,B). Scale bar in (C) for panels (C-F) is 50 μm. * in (C,D) shows the location of the interior of the optic vesicle prior to invagination of the optic cup. D, dorsal; V, ventral; ba, branchial arches; h, heart; L, lens; nr, neural retina; ot, otic vesicle; rpe, retinal pigment epithelium. |
