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Fig. 3. Acinar cells transdifferentiate to duct-like cells in a transgenic pancreas induced to express ST3. (See A of Fig. 2 for the two constructs). The sections are double labeled with
antibodies against carbonic anhydrase-II (CA-2) (green) a specific duct cell marker, and amylase (red) an acinar-specific marker. A) premetamorphic control tadpole, B) control adult
frog. A premetamorphic (NF55) transgenic tadpole was induced with tetracycline for C) 2 days, D) 4 days, and E) 8 days. The dotted square in D is magnified in F and G to
demonstrate that CA-2 and amylase are expressed in the same cells. At 8 days of exposure amylase can no longer be detected, and the total number of cells has decreased. The arrows
indicate cells that express both amylase and carbonic anhydrase. Scale bar 20 μm. |
