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Fig. S1. casp3 and barhl2 are coexpressed in the stage (st) 26 prosomere P2. (A–C) ISH with casp3 antisens (A and C) or casp3 sens (B) probes on st 26 dissected neural tubes. (A and B) At st 26, casp3 is mostly expressed in the telencephalic prospective pallium, in the diencephalic and mesencephalic alar plates of the neural tube, and in the pineal gland. (C) Enlargement of the prosomere P2 area of A. barhl2 expression domain is indicated with a black line. (D) RT-PCR analysis on RNA purified from telencephalic (TEL) or diencephalic (DIEN) st 26 neural tube parts. Foxg1 and barhl2 are pallial TEL and DIEN markers, re- spectively. Histone H4 and the plasmid encoding Casp3 were used as positive controls, and water was a negative control (ct). |