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Fig. 2. (A–E) Whole-mount in situ hybridization on Xenopus embryos using Xcalp1 as probe: (A) stage 20 (B) stage 24 (C) stage 28 (D) stage 36 (E) enlargement of the caudal region of the embryo showed in (D). (F) Whole-mount in situ hybridization using Xcalp3 as probe: transverse section of a stage 26 embryo. (G) RT-PCR analysis of Xcalp1, 2, 3 mRNA expression in different Xenopus embryonic stages and in the adult brain. An example of −RT control is shown. Band sizes are indicated in base pairs. The histone H4 is used for normalization. |