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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
cbfb	xenopus		neuron

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Experiment details for cbfb

Expression analysis of Runx3 and other Runx family members during Xenopus development.

Expression analysis of Runx3 and other Runx family members during Xenopus development.

Gene	Clone	Species	Stages	Anatomy
cbfb.S		laevis	NF stage 13 to NF stage 40	Rohon-Beard neuron

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Fig. 3. Comparative analysis of the expression of Runx and Cbfβ genes by whole-mount in situ hybridization. Runx3 is first detected at stage 20 in two domains, the developing primary sensory (Rohon-Beard) neurons and the trigeminal-profundal placode. At the same stage Cbfβ is also co-expressed in both domains, while Runx1 is restricted to Rohon-Beard neurons. At later stages, stage 25 and up, Runx3 expression appears to be largely confined to the head region and in a number of cranial nerves including the ganglion of the trigeminal, vagal and glossopharyngeal nerves. To confirm the tissue-specific expression of Runx genes, we also performed in situ hybridization for Pax3 and Islet1 in staged matched embryos. Pax3 and Islet1 share common expression domains with Runx genes in the profundal-trigeminal placode (Pax3 and Islet1), Rohon-Beard sensory neurons and cranial ganglia (Islet1). Stages are according to Nieuwkoop and Faber (1967). Stage 13, dorsal view anterior to top. Stage 20d, dorsal view anterior to the left. Stage 20f, frontal view dorsal to top. Stage 25–40, lateral view of the head region, anterior to the left and dorsal to top. ba, branchial arches region; bp, blood progenitors; hf, heart field; hg, hatching gland; gp, glossopharyngeal; nb, neural plate border; ol, olfactory placode; pr, profundal ganglia; rb, Rohon-Beard neurons; sa, statoacoustic ganglia; tg, trigeminal ganglia; pt, profundal-trigeminal placode; va, vagal nerve. The scale bars in the upper row represent 500 μm.

Gene	Clone	Species	Stages	Anatomy
cbfb.S		laevis	NF stage 20	Rohon-Beard neuron , neuron

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Fig. 5. Comparative analysis of Runx3, Runx1, Cbfβ and Islet1 expression in the profundal-trigeminal placode and Rohon-Beard sensory neurons at stage 20. (A) Schematic representation of a stage 20 embryo view from the dorsal side, anterior to the left. Modified from Nieuwkoop and Faber (1967). (B) Schematic representation of a transverse section at the level of the diencephalon, corresponding to the line labeled “B” in panel (A). Dorsal to top. The blue areas indicate the position of the developing profundal-trigeminal placode. The boxed area labeled “C” is the region of the section shown in subsequent panels (C). (C) Runx3, Cbfβ and Islet1 are co-expressed in the profundal-trigeminal placode (blue arrows), while Runx1 is not detected in this tissue. (D) Transverse sections in the trunk region corresponding to the line labeled “D” in panel (A). All four genes are co-expressed in Rohon-Beard sensory neurons (red arrows). Islet1 is also expressed in the population of ventral interneurons (black arrows). The notochord is outlined with a solid line, while the position of the neural tube is underlined with dashed lines. The probes are indicated in the lower right corner of each panel. di, diencephalon; no, notochord; ov, optic vesicle. The scale bar in panel C represents 100 μm.