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Fig. 7. KLF2 is necessary and sufficient for expression of other vascular marker genes. In situ hybridization analysis of X-msr and VE-cadherin expression in injected Xenopus embryos (stage 34, lateral view). (A-D) Klf2 MO blocks expression of the vascular markers X-msr and VE-cadherin, as compared with control MO-injected embryos. The insets are higher magnification views, centered on the developing posterior cardinal vein. (E-H) Injection of Klf2 mRNA (500 pg) induces ectopic expression of X-msr and VE-cadherin (arrows). |