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cdknxxenopus ventral 

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Experiment details for cdknx

Movassagh M and Philpott A (2008) Assay

Cardiac differentiation in Xenopus requires the cyclin-dependent kinase inhibitor, p27Xic1.

Gene Clone Species Stages Anatomy
cdknx.L laevis NF stage 16 to NF stage 17 ventral
cdknx.L laevis NF stage 18 ventral

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  Figure 6. Xic1 is required for late stages of cardiomyocyte differentiation. Expression of Nkx2.5 (A) or Xic1 (B) by in situ hybridization at described stages. Nkx2.5 staining is largely localized to the ventral side of embryos in the cardiac crescent (A, white arrows). In contrast, Xic1 expression is predominantly dorsal (B, Dorsal view, black arrows) and only weakly detected at the ventral side (B, ventral view, black star-arrowhead). (C) Xic1Mo does not affect the cardiac field as measured by Nkx2.5 expression at stages-17, -19 and -21 of development. (D) RT–PCR from uninjected embryos or embryos injected with either CTRMo or Xic1Mo to detect expression of TIc and MHCα with ODC as loading control, stages as described. (E) Quantitative RT–PCR demonstrating the relative expression of xMLC1v in embryos described in (D), following normalization against xODC.

Gene Clone Species Stages Anatomy
cdknx.L laevis NF stage 31 ventral
cdknx.L laevis NF stage 33 and 34 ventral

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  Figure 3. Xic1 is expressed in the differentiating heart. In situ hybridization to detect Xic1 in ventral or lateral view (A–E) of stage 27–41 embryos. Xic1 is weakly expressed in the hearts of embryos from stage-27 onwards (arrows). Picture B was taken following clearing embryo with Benzyl-benzoate:Benzyl-alcohol. (G) RT–PCR confirming expression of Xic1 transcript in the dissected hearts (F) of stages-33/4 to -42.