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Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
nkx2-5
|
|
laevis
|
NF stage 14
|
mesoderm
,
cardiac mesoderm
,
ventral
,
cardiac progenitor cell
|
nkx2-5
|
|
laevis
|
NF stage 15
|
mesoderm
,
cardiac mesoderm
,
ventral
|
nkx2-5
|
|
laevis
|
NF stage 16
to
NF stage 17
|
mesoderm
,
cardiac mesoderm
,
ventral
|
nkx2-5
|
|
laevis
|
NF stage 18
|
mesoderm
,
cardiac mesoderm
,
ventral
|
nkx2-5
|
|
laevis
|
NF stage 19
|
mesoderm
,
cardiac mesoderm
,
heart primordium
|
nkx2-5
|
|
laevis
|
NF stage 21
|
mesoderm
,
cardiac mesoderm
,
heart primordium
|
cdknx
|
|
laevis
|
NF stage 14
|
neural plate
,
dorsal
,
neural plate border
|
cdknx
|
|
laevis
|
NF stage 15
|
neural plate
,
dorsal
,
neural plate border
|
|
|
Figure 6. Xic1 is required for late stages of cardiomyocyte differentiation. Expression of Nkx2.5 (A) or Xic1 (B) by in situ hybridization at described stages. Nkx2.5 staining is largely localized to the ventral side of embryos in the cardiac crescent (A, white arrows). In contrast, Xic1 expression is predominantly dorsal (B, Dorsal view, black arrows) and only weakly detected at the ventral side (B, ventral view, black star-arrowhead). (C) Xic1Mo does not affect the cardiac field as measured by Nkx2.5 expression at stages-17, -19 and -21 of development. (D) RT–PCR from uninjected embryos or embryos injected with either CTRMo or Xic1Mo to detect expression of TIc and MHCα with ODC as loading control, stages as described. (E) Quantitative RT–PCR demonstrating the relative expression of xMLC1v in embryos described in (D), following normalization against xODC. |
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Display additional annotations [+]
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Figure 3. Xic1 is expressed in the differentiating heart. In situ hybridization to detect Xic1 in ventral or lateral view (A–E) of stage 27–41 embryos. Xic1 is weakly expressed in the hearts of embryos from stage-27 onwards (arrows). Picture B was taken following clearing embryo with Benzyl-benzoate:Benzyl-alcohol. (G) RT–PCR confirming expression of Xic1 transcript in the dissected hearts (F) of stages-33/4 to -42. |