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Figure 1. Expression of Cfap161 in mouse and Xenopus. (A) Analysis of Cfap161 and Foxj1 expression by RT-PCR of RNA from wild type adult organs as indicated on top. Hprt was used as quality control. The full-size agarose gel is shown in Fig. S8. (B) WISH of E7.75 wild type murine embryo (a, a′) and SISH of E16.5 wild type embryos showing Cfap161 expression (arrowheads) in the node (a) and indicated tissues (b–e) that develop motile cilia. Red boxed area in (a) indicate the region shown in higher magnification in (a′). (C) SISH analysis of wild type adult tissues (indicated on top). Red boxed areas in (a–f) indicate the regions shown at higher magnification in (a′–f′). White arrowheads point to regions of expression in cells possessing motile cilia. Red arrowheads point to regions of cells with primary cilia. PRL photoreceptor layer, INL inner nuclear layer, GCL ganglion cell layer. (D) Xenopus WISH detected cfap161 transcripts in the left–right organiser (LRO; a, a′), floor plate (FP; a′), nephrostomes marked by white arrowheads (b), multiciliated cells (MCCs) of the skin (b), stomach (stom.; c, c′), branchial chamber (BC; c, c″) subcommissural organ (SCO; c, c‴) and in the zona limitans intrathalamica (ZLI; c, c‴). Transversal section planes are indicated with stippled lines and shown in (a′, c′, c″, c‴). Scale bars: (B, D) = 100 µm; (C) = 500 µm. |
