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Figure 7 cnr1 knockdown leads to eye defects. (A) qRT-PCR analysis of cnr1 mRNA expression during Xenopus laevis development.
The expression level of cnr1 was normalized to that of odc. The bar graph indicates the ratio of the normalized expression level
in each stage to that in stage 33/34, from each of two independent experiments. (B) Whole-mount in situ hybridization for cnr1
mRNA. The representative results from two independent experiments are shown. Anterior is upwards in the dorsal view and leftwards
in the lateral views. Dorsal is upwards in lateral and anterior views. The lateral view of a stage 33/34 embryo is magnified in
the lower middle panel. e, eye; br, brain. (C, D) Control MO, cnr1 MO1, cnr1-5mis MO or cnr1 MO2 (20 ng each/blastomere)
was injected into the animal region of two dorsal blastomeres at the 4-cell stage. Injected embryos were cultured until stage 41. Lateral
views with anterior leftwards and dorsal upwards. Representative results from three independent experiments are shown. (C)
Embryos injected with either control MO or cnr1-5mis MO developed normally (control MO, 100%, n = 60; cnr1-5mis MO,
100%, n = 60), whereas those injected with cnr1 MO1 displayed eye defects (100%, n = 60). (D) Embryos injected with control
MO developed normally (100%, n = 60), whereas those injected with cnr1 MO2 displayed eye defects (100%, n = 60). |
