|
Display additional annotations [+]
|
| |
Figure 6. Expression of RA-modifying genes during hindlimb regeneration. Dark blue stain indicates expression, white arrowheads mark the level of amputation; tissue to the right of this is regrowth. A–C:Cyp26b is expressed strongly in mesenchymal blastema cells, but is absent from the AEC. D–F:Crabp2 is also strongly upregulated in the blastema although it appears absent from the distal tip (white arrow in E). D′–F′: Expression of Crabp2 in non-regenerating, transgenic N1 tadpole hindlimbs to show residual expression. G–I:Raldh2 is not expressed in regenerating tissue but note that it is expressed in the anterior stump tissue (marked with a white asterisk) providing a potential source of RA to the blastema. Limbs are viewed from the dorsal side and oriented with proximal to the left and posterior uppermost, the tadpole's head is to the left. Scale bar in I = 0.5 mm (applies to all panels). |
