Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Search Criteria
Gene/CloneSpeciesStageAnatomy ItemExperimenter
dll1xenopus dorso-lateral 

Too many results?Too few results?

Experiment details for dll1

Kuriyama S et al. (2006) Assay

Tsukushi controls ectodermal patterning and neural crest specification in Xenopus by direct regulation of BMP4 and X-delta-1 activity.

Gene Clone Species Stages Anatomy
dll1.L laevis NF stage 17 dorso-lateral

Display additional annotations [+]
  Fig. 6. X-TSK interacts with the Notch signaling pathway. Whole-mount in situ hybridization analysis of mRNA-injected embryos. Injected samples are indicated in the upper right-hand corners; probes are indicated in the lower right-hand corner. (A,C) Neural crest markers Xslug (A) or Sox9 (C) disappeared or diminished (arrowheads) with X-TSK (1 ng) overexpression. (B) The β-gal mRNA (0.5 ng) injection had no effect. (D) X-TSK-CD2 mRNA (1 ng) showed a similar effect to that in C. (E) Sox2 expression expanded laterally (right bar) in the X-TSK (1 ng)-injected side. (F) Epidermal keratin levels diminished. The anterior border disappeared on the injected side (open arrowhead). (G) An embryo injected with Chordin (100 pg). The Sox2-expressing neural plate is expanded (right bar). (H) An embryo injected with Chordin (100 pg). Expression of the neural crest marker Sox9 diminished (arrowhead). (I,J) X-TSK-si (0.5 pmol) and Chordin mRNA (100 pg). Faint expression of Sox9 (I) or Xslug (J) was observed in the far lateral side (arrowheads). (K) Hairy2A expression is downregulated on X-TSK-injected side, though it appears to spread over a broader domain (arrowhead and open arrowhead). (L) X-TSK mRNA (1 ng) injection resulted in decreased expression of Msx-1 (arrowhead) though the width of its expression area (right bracket) becomes wider than in the control side. (M) An embryo injected with X-TSK (1 ng). Xbmp4 expression increased outside the injected side (arrowhead). (N) An embryo injected with Chordin (100 pg). Xbmp4 expression disappeared around the β-gal-positive cells (arrowhead). (O) BMP4 (0.5 ng) mRNA and (P) Notch ICD (0.5 ng) injections caused reduction of Xslug expression (arrowheads). (Q) β-Gal mRNA injection did not affect Xbmp4 expression. (R) X-delta-1Stu injected areas are marked by β-gal staining (light blue), the arrowhead indicates ectopic Xbmp4 expression (dark purple). (S,T) An embryo injected with X-TSK. (S) Two out of the three strips of N-tubulin expression disappeared (arrowheads). (T) X-delta-1 expression disappeared on the outer border of the neural crest (arrowhead). (U) β-gal did not affect X-ESR-1 expression. (V) X-TSK mRNA (1 ng) injection resulted in expanded peripheral expression of X-ESR-1 on the injected side (arrowheads). (W,X) Co-injection of 1 ng X-TSK and 0.5 ng Su(H)DBM mRNAs. (W) Diffused signals of Sox9 were observed around the arrowhead. (X) Xbmp4 expression was extended towards theβ -gal-positive side (arrowhead). (Y) Sox9 expression was reduced in Su(H)/Ank 500 pg injected side (arrowhead). (Z) Sox9 expression was completely missing in X-TSK 1 ng+ Su(H)/Ank 500 pg injected side (open arrowhead). (AA) Xbmp4 expression was very weakly inhibited in Su(H)/Ank 500 pg injected side (open arrowhead). (BB) The patchy expression of Xbmp4 was observed in X-TSK 1 ng+ Su(H)/Ank 500 pg injected side (arrows). The expansion of Xbmp4-expressing regions was not observed (open arrowhead).