| |
Figure 4. Expression of selected clones during cornea-lens transdifferentiation. mRNA expression was examined by means of in situ hybridization 24, 48, 72, and 96 hr after lentectomy in tadpoles at stages 47-51. Representative examples and regeneration times are shown as follows: A, W001 at 48 hr; B, W002 at 24 hr; C, W003 at 24 hr; D, W004 at 24 hr; E, W007 at 24 hr; F, W011 at 96 hr. White dotted lines illustrate the approximate boundary of staining in the cornea epithelium. Open arrowheads in B and F indicate prominent staining of lateral line organs by probes W002 and W011, respectively. G: The contralateral, unoperated lens from F (probed with W011). H: An eye 96 hr after lentectomy probed with a ldquo sense rdquo control riboprobe for W011. All specimens were examined during developmental stages 47-51 (Nieuwkoop and Faber, [1956]); eye morphology does not demonstrably differ during these stages. Scale bar = 300 mu m in H (applies to A-H). |
