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Fig. 2. Comparison of ELGs expressions in the brain. Double in situ hybridizations. (A-I) Double in situ hybridizations were performed on sections of stage 40 Xenopus embryos at the level of the brain. Expressions of elrC (dark blue) and elrD (red) are compared on the same section of the hindbrain (A-C) and at the level of the pineal gland (D-F). (A,D) elrC is mainly expressed in the ventricular zone and in the dorsal region of the brain. (B,E) elrD expression is found in the cortical plate, most strongly in the ventral part, and in the pineal gland (arrow in E). (C,F) Double exposure: where elrD is strongly expressed, elrC is expressed at a low level and conversely where elrC is most strongly expressed, elrD is not detected. For instance, elrD is expressed in the pineal gland (arrow) whereas elrC is not (F). (G-I) elrD (dark blue) and elrB (red) expressions on the same section of the hindbrain. elrD (G) and elrB (H) are both expressed in the cortical plate, and most strongly in the ventral part. (I) Double exposure: elrB and elrD expression domains largely overlap. (J-O) Comparison of ELGs and islet-1 expressions. In situ hybridizations for elrD, elrC and elrB (dark blue staining), followed by immunohistochemistry using anti-islet-1 antibody (red staining), were performed on cryostat brain sections of stage 40 Xenopus embryos. Islet-1 positive cells are present in the most ventral part of the cortical plate (K, M, O, arrow). Faint cytoplasmic dorsal red labeling reflects non-specific staining. (J,K) Comparison of elrD and islet-1 expressions on the same section of the midbrain. elrD expression domain overlaps that of islet-1, however elrD domain is broader and extends more dorsally. (L,M) Comparison of elrC and islet-l expression on the same section of the midbrain. Note that a low level of elrC transcripts (L) in the ventral region of the cortical plate correlates with expression of islet-1 (M). (N and 0) Comparison of elrB and islet-1 expression on the same section of the midbrain. elrB is expressed, albeit at a low level, in the ventral region of the cortical plate (N). The most ventral expression of elrB partially overlaps that of islet-1. Scale bar, 30 um. |
