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Fig. 4. Comparison of N-tubulin and ElrC expression in the neural plate and neural tube. (A–D) Neural plate stage (stage 16) embryos hybridised with the markers indicated, some are additionally stained for proliferation by BrdU incorporation (brown nuclei; C, D). Arrows point to BrdU positive cells that express ElrC but not N-tubulin. Arrowhead points to an example of a BrdU positive cell that does not express ElrC. (E, F) Sections of tailbud neural tube (stage 23) at the level of the otic vesicle (ov), stained as indicated. Inset is a section demonstrating the location of BrdU in the neural tube. (G, H) Tadpole neural tube section (stage 34), stained as indicated. In all stages, the overlap between ElrC and N-tubulin expression is partial. At the neural tube stage, note that ElrC+ cells are located closer to the ventricle than N-tubulin+ cells. |
