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Fig. 7. xMIDs function in the developing brain and other epithelial organogeneses. (A-J) Transverse sections through the brain (A,B), optic vesicle (C,D), cement gland (E,F), pronephros (G,H) and foregut (I,J) of Xenopus embryos injected with control-Mo (A,C,E,G,I) or xMID-Mo (B,D,F,H,J), and stained with phalloidin (green), anti-laminin antibody (magenta), or anti-Flag antibody (gray). (A,B) Insets show stage 40 embryos stained for the pan-neural marker Xen1. Lateral views: anterior to the left, dorsal to the top. Arrowheads indicate head defects in the xMID morphant. (B,D) Arrowheads indicate non-continuous and attenuated laminin staining in the basal lamina. (E,F) Brackets indicate the lengths of Flag-positive cells. (G) Asterisks indicate tubular structures of the pronephros. (I,J) Asterisks indicate luminal structures of the foregut. Arrowheads indicate the lack of apical actin assembly in the xMID morphant cells. (K,L) Transverse sections through the brain of control-Mo-injected (K) and xMID-Mo-injected (L) embryos, stained with anti-active caspase 3 (left) and anti-Flag (right) antibodies. Dashed line delimits the ventricle. |
