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FIGURE 10. Analysis of progenitor cells at larval stages (Tbr2, BLBP, Pax6, PH3). Micrographs of transverse sections through the pallium of Xenopus laevis at the larval stage 54 showing the distribution of the markers Tbr2 (A,C,D′,E′,F,K), BLBP (B,C,D,E) and Pax6 (G–J,L), and the combination of Tbr2 with the mitotic marker PH3 (M–P). In each panel the color code for the used markers is indicated. The higher magnifications (D,E) correspond to the framed areas, as indicated (white boxes in A,B). Tbr2 expressing cells were detected close to the ventricle and away from it (A,D′,E′,F,K) but only those cells in the ventricular zone expressed BLBP (D,D′,E,E′) or Pax6 (F–L). The combination of Tbr2 and PH3 showed that there are not Tbr2 mitotic cells away from the ventricle (M–P). Scale bars = 200 μm (A,B,F,G), 100 μm (C,H,J–M), 50 μm (D,E,I,N,O,P). See list for abbreviations. |
