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Fig. 3. Late gene expression in the Xenopus tail bud. Whole-mount in situ hybridisations to show expression of late genes in the tail bud during early extension of the tail. (A), Xwnt3a; (E), lunatic fringe; (I), Xhox3. (A,E,I) show the expression patterns at stage 30 viewed from the left side with anterior to the left, tail bud to the right and dorsal up. (B,F,J) show detail of the tail bud expression at stage 30, in cleared specimens. (C G,K) are sagittal sections through stage 30 embryos. Scale bar, 250 mm. Note the sharp posterior boundaries of lunatic fringe and Xwnt3a expression in the dorsal roof, and restriction of Xhox3 to the distal tip of the posterior wall, (D,H,L) are schematic representations of (C,G,K), respectively. (M) Stage 30 tail bud showing BMP-4 expression in the fin and epidermis. (N) X-serrate-1 expression in the fin. Abbreviations: cnh, chordoneural hinge; end, endoderm; nc, notochord; nt, neural tube; pw, posterior wall. |
