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(A) Ubiquitous distribution of maternal ezrin transcripts at 512-cell-stage. (B) At 60 % epiboly stage, ezrin is expressed ubiquitously. (C) At the 3-somite stage, expression of ezrin mRNA is restricted to the polster (black arrowhead). (D) At the 20-somite stage ezrin shows expression in the otic vesicle (black arrowhead) and the developing pronephric tubule (black arrow). (E) At 22 hpf ezrin expression is detected in the forebrain (black arrow), epiphysis (black arrowhead), otic vesicle (white arrowhead) and the pronephric tubule (white arrow). Cross-section of a 36 hpf embryo (F) shows that ezrin (blue) partially co-localizes with cadherin 17 (red) in the pronephric tubule. (G) At 48 hpf, ezrin has a broad expression throughout the brain, epiphysis (black arrowhead) and pronephric tubule (white arrow). (H) Immunoblot of 24 hpf zebrafish lysates after injection of ezrin mRNA indicates a strong upregulation of Ezrin expression in comparison to control embryos. Anti-γ-Tubulin
immunoblot served as loading control. (I) ezrin is expressed in the cement gland (white arrow), otic vesicle (white arrowhead), pronephros (black arrow), cloaca (black arrowhead) and throughout the epidermis at stage 32 of Xenopus development. (J) Immunoblot of Xenopus lysates (stage 32) after injection of TB-Mo ezrin (8 ng) into the animal-ventral blastomeres of four-cell stage Xenopus embryos indicates a reduction of Ezrin expression compared to Co-MO (8 ng) injected embryos. Anti-γ-Tubulin immunoblot served as loading control. Scale bars, 100 μm (A,B,C,D,E,G), 20 μm (F), 500 μm (I).
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