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fermt1xenopus ectoderm [+] 

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Experiment details for fermt1

Developmental expression of the fermitin/kindlin gene family in Xenopus laevis embryos.

Developmental expression of the fermitin/kindlin gene family in Xenopus laevis embryos.

Gene Clone Species Stages Anatomy
fermt1.S laevis NF stage 10.5 ectoderm

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  Figure 2. Expression of Fermt1 and Fermt2 at gastrula stages. A, B: Expression of both Fermt genes is detected throughout the gastrula embryo. Ectoderm, endoderm, and mesoderm were isolated from embryos at stage 10.5 and analysed for Fermt1 and Fermt2 expression by RT-PCR (C). Fermt1 is expressed in the ectoderm (lane 1) and mesoderm (lane 2) and more weakly in the endoderm (lane 3). Fermt2 is expressed in all three germ layers at stage 10.5. ODC serves as a loading control.

Gene Clone Species Stages Anatomy
fermt1.S laevis NF stage 12 to NF stage 18 ectoderm
fermt1.S laevis NF stage 22 ectoderm
fermt1.S laevis NF stage 28 otic vesicle , midbrain , stomodeal-hypophyseal primordium , dorsal fin
fermt1.S laevis NF stage 35 and 36 otic vesicle , forebrain , olfactory bulb , skin , dorsal fin

  Figure 3. Expression of Fermt1 from stages 12 to 35. A:Fermt1 expression at stage 12, 15, and 18, respectively. Expression in the ectoderm is clearly seen in transverse section. D: Fermt1 expression is detected throughout the ectoderm and weakly in the somites at stage 22. E: At stage 28, Fermt1 becomes restricted to a number of ectodermal derivatives, including otic, olfactory, and pharyngeal placodes, and pronephric anlage. F: By stage 35, Fermt1 transcripts are detected in the otic vesicle (black arrow), olfactory pit (black asterisk), heart (back arrowhead), and in the pronephric duct (white arrowhead). G: Transverse sections are observed through the level of the eye/olfactory pit (G), the otic vesicle (H), and the pronephric duct (I). Inset (F) demarcates the levels at which sections were analysed (G).

Gene Clone Species Stages Anatomy
fermt1.S laevis NF stage 35 and 36 otic vesicle , forebrain , olfactory bulb , dorsal fin

  fermt1 (FERM domain containing kindlin 1) gene expression in Xenopus laevis embryos, NF stage 35, as assayed by in situ hybridization, lateral view,anterior left, dorsal up.