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Fig. 1. Gene expression profiles during Xenopus tadpole tail regeneration. (A–X) In situ hybridization detection of fgf8 (A–D), fgf9 (E–H), fgf10 (I–L), fgf20 (M–P), wnt3a (Q–T) and wnt5a (U–X) in control tadpoles (A, E, I, M, Q, U; stage 42–43 tadpoles) and in tail regenerates 1–3 days post amputation (1 dpa: B, F, J, N, R, V; 2 dpa: C, G, K, O, S, W; 3 dpa: D, H, L, P, T, X; stage 48+ tadpoles). Arrowheads indicate amputation levels. Scale bars: 200 μm. dpa: day post amputation. (Y) RT-PCR detection of FGF, BMP and Wnt signalling components in tadpole tail immediately after amputation (D0) and 1–4 days post amputation (D1–D4). 1 mm of stump tissue was used to prepare RNA. ODC (ornithine decarboxylase) is used as loading control. (Z) Relative expression levels of genes detected by RT-PCR, based on the density of the PCR bands in (Y). The band density on D0 is the reference standard (1 arbitrary unit) in each gene group. Note that an increased in situ signal may correspond to a limited overall change in the larger tissue region used for RNA analysis. |
