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Fig. 3. Whole mount in situ hybridization
of developing Xenopus
embryos for Fmr1. (A) Early
tailbud stage embryo (st. 26) showing
Fmr1 expression in the CNS
(white arrowheads) and pharyngeal
arches (black arrowheads). (B) Expression
of AP-2α, a neural crest
marker at tailbud stage (st. 26). In
comparison to Fmr1 expression
pattern, AP-2α expression is mostly
limited to the pharyngeal arches at
this stage (black arrowheads). (C)
Fmr1 expression at early tadpole
stage embryo (st. 26; see F and G
for details). (D) Horizontal section of
st. 26 embryo at the level of cement
gland showing Fmr1 expression in
endodermal (black arrowhead),
mesodermal (black arrow) and mesenchymal
(white arrowhead) regions
of the pharyngeal arches, with slight
staining in the ectodermal region.
The difference in staining intensity
between the left and right side is due to the oblique nature of the sectioned tissue. Cement gland (cg) is negative for Fmr1 expression. (E) Oblique
transverse section of tailbud stage embryo (st. 26). Strong expression is observed in the eye evagination (ey) and in CNS (white arrowhead). Insets show
approximate region that has been sectioned (mg: midgut). (F,G) Sagittal section of late tailbud stage embryo depicted in Fig. 3C showing strong expression
in the CNS (G: white arrowhead), craniofacial regions, notochord (F,G: black arrowheads), ear vesicle (F: ev) and eye (G: ey). |
