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Fig. 1. Effects of anti-Xanf MO on expression of different marker genes in the neural plate of Xenopus laevis midneurula embryos (stage 14). (A–J) Expression of Hox9, Wnt8, Otx2, Pax6, Six3 and Engrailed2 (in situ hybridization with the mixture of probes), Xanf, Bf2 and Engrailed2 (in situ hybridization with the mixture of probes), Bf1, Fgf8 and Xanf respectively in control embryos. (A1–J1) Expression of the aforementioned markers in embryos microinjected with anti-Xanf MO. Note the anterior expansion of expression of the posterior forebrain markers (Otx2 and Pax6) in contrast to inhibition of the rostral ones (Bf1, Bf2, Fgf8). (A1′–J1′) Distribution of FLD tracer in embryos shown in panels A1–J1 is shown in fluorescence. (A2–J2 and A3–J3) Schematic diagrams of expression domains (purple) of the aforementioned marker genes in control (A2–J2) and experimental (A3–J3) embryos are shown in comparison with the normal expression domain of Xanf (pink). All embryos are shown from the anterior, dorsal side up. Approximate position of the anterior margin of the neural plate is indicated by the red dotted line. |
